Folate status, folate-related genes and serum miR-21 expression: Implications for miR-21 as a biomarker

被引:22
|
作者
Beckett, Emma Louise [1 ,2 ]
Martin, Charlotte [1 ]
Choi, Jeong Hwa [1 ]
King, Katrina [3 ]
Niblett, Suzanne [3 ]
Boyd, Lyndell [1 ]
Duesing, Konsta [2 ]
Yates, Zoe [4 ]
Veysey, Martin [3 ]
Lucock, Mark [1 ]
机构
[1] Univ Newcastle, Sch Environm & Life Sci, Brush Rd,POB 127, Ourimbah, NSW 2258, Australia
[2] CSIRO, Food & Nutr Flagship, N Ryde, NSW 2113, Australia
[3] Teaching & Res Unit, Gosford 2250, Australia
[4] Univ Newcastle, Sch Biomed Sci & Pharm, Ourimbah, NSW 2258, Australia
来源
BBA CLINICAL | 2015年 / 4卷
关键词
MiR-21; MicroRNA; Adenomatous polyps; Colorectal cancer; Folate;
D O I
10.1016/j.bbacli.2015.06.006
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: Free circulating microRNA (miRNA) in serum may be valuable biomarkers for disease diagnosis and prognosis. miR-21, the archetypal oncogenic miRNA, has been proposed as a biomarker for colorectal cancer and its benign precursor, adenomatous polyps. However, it is now becoming clear that circulating miRNA profiles may be sensitive to lifestyle and environmental influences. Dietary components involved in one-carbon metabolism are particularly well placed to modulate miRNA expression through an influence on DNA methylation pathways. Methods: We investigated the role of methyl group donors (folate, B12, cysteine, homocysteine), polymorphisms of the enzymes of one-carbon metabolism, and serum miR-21 expression in a primary case-control cohort (colonoscopy confirmed adenomatous colon polyps vs controls; n = 253) and a secondary cross-sectional cohort (over 65s; n = 649). The relationships between these parameters and serum miR-21 levels were assessed, stratified by gender. Conclusions: Serum miR-21 expression was related to occurrence of adenomatous polyps in females, but not males. Folate levels and MTHFR-C677T genotype was associated with miR-21 expression in both genders. Additionally, DHFR-19 del and MSR-A66G were associated with miR-21 expression in females and males, respectively. Stimulation with excess folate increased expression of miR-21 in colon cancer cell lines. General significance: This study demonstrates that serum miR-21 expression correlates with folate status and related genetic status. This may have consequences for the proposed use of miR-21 as a colorectal cancer biomarker. Crown Copyright (C) 2015 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码:45 / 51
页数:7
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