SYNTHESIS AND USE OF A NOVEL N-FORMYL PEPTIDE DERIVATIVE TO ISOLATE A HUMAN N-FORMYL PEPTIDE RECEPTOR CDNA

被引:266
作者
BOULAY, F
TARDIF, M
BROUCHON, L
VIGNAIS, P
机构
[1] LBIO/Laboratoire de Biochimie, Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, 38041 Grenoble Cedex
关键词
D O I
10.1016/0006-291X(90)91143-G
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
N-formyl-methionyl peptides are powerful chemoattractants which bind to specific receptors on the neutrophil plasma membrane. A cDNA library from HL-60 cells, differentiated into granulocytes highly responsive to N-formyl-methionyl peptides, was constructed in the COS cell expression vector CDM8. A cDNA clone was isolated that conferred to COS cells the ability to bind a new and highly efficient hydrophilic derivative of N-formyl-Met-Leu-Phe-Lys. The transfected COS cells displayed two classes of binding sites with Kd values of 1-5nM and 5-10 nM, respectively. The cDNA was 1.9 kb long with a 1050 bp open reading frame encoding a 350 residue protein. The hydropathy plot analysis revealed seven hydrophobic segments, a pattern quite similar to that of G protein-coupled receptors. © 1990.
引用
收藏
页码:1103 / 1109
页数:7
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