SEPARATION AND CHARACTERIZATION OF CYTOKININ-INDUCIBLE (S)-TETRAHYDROBERBERINE OXIDASES CONTROLLING BERBERINE BIOSYNTHESIS IN THALICTRUM-MINUS CELL-CULTURES

被引：9
作者：
HARA, M
MORIO, H
YAZAKI, K
TANAKA, S
TABATA, M
机构：
[1] Faculty of Pharmaceutical Sciences, Kyoto University, Yoshida, Kyoto
来源：
PHYTOCHEMISTRY | 1995年 / 38卷 / 01期
关键词：
THALICTRUM MINUS; RANUNCULACEAE; PLANT CELL CULTURE; CYTOKININ; 6-BENZYLAMINOPURINE; TETRAHYDROBERBERINE OXIDASE; BERBERINE BIOSYNTHESIS;
D O I：
10.1016/0031-9422(94)00623-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Three forms of cytokinin-induced tetrahydroberberine (THE) oxidase from Thalictrum minus cell suspension cultures were individually separated by chromatography using DEAE-TOYOPEARL. Two of them were (S)-THB-specific oxidases, whereas the third was found to be a non-stereospecific one. The stereospecific oxidases were different from each other not only in the K-m value, but also in the time course of activation induced by 6-benzylaminopurine (BAP). The substrate specificity of these oxidases from T. minus cells was more similar to that reported for the (S)-THB oxidase of Coptis japonica than that for Berberis stolonifera cell cultures.
引用
收藏
页码：89 / 93
页数：5
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