DETERMINATION OF L-365,260, A NEW CHOLECYSTOKININ RECEPTOR (CCK-B) ANTAGONIST, IN PLASMA BY LIQUID-CHROMATOGRAPHY ATMOSPHERIC-PRESSURE CHEMICAL IONIZATION MASS-SPECTROMETRY

被引：44

作者：

GILBERT, JD ^{[1
]}

HAND, EL ^{[1
]}

YUAN, AS ^{[1
]}

OLAH, TV ^{[1
]}

COVEY, TR ^{[1
]}

机构：

[1] SCIEX LTD,TORONTO L3T 182,ONTARIO,CANADA

来源：

BIOLOGICAL MASS SPECTROMETRY | 1992年 / 21卷 / 02期

关键词：

D O I：

10.1002/bms.1200210202

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

L-365,260 is a novel cholecystokinin receptor antagonist. A sensitive and specific liquid chromatographic/mass spectrometric assay has been developed for the determination of the drug in plasma using the CD3-labeled species as the internal standard. Plasma extracts were separated on a 3 cm C-18 reverse-phase high-performance liquid chromatography column. The column eluate passed, by means of a heated nebulizer interface, into a corona discharge atmospheric chemical ionization source. The mass spectrometer was operated in the positive ion tandem mass spectrometric mode. The method has sufficient sensitivity, specificity, precision, accuracy and selectivity for the determination of drug concentrations in clinical samples. The chromatographic run time is less than 2 min.

引用

页码：63 / 68

页数：6

共 20 条

[1]

ABELSON JL, 1990, ARCH GEN PSYCHIAT, V47, P395

[2] BENZODIAZEPINE GASTRIN AND BRAIN CHOLECYSTOKININ RECEPTOR LIGANDS - L-365,260 [J].