EFFECT OF ALPHA-KETOGLUTARATE AND ITS STRUCTURAL ANALOGS ON HYSTERETIC PROPERTIES OF ALPHA-KETOGLUTARATE DEHYDROGENASE

被引：12

作者：

BUNIK, VI

ROMASH, OG

GOMAZKOVA, VS

机构：

[1] Department of Biochemistry, Moscow State University, Moscow

来源：

FEBS LETTERS | 1991年 / 278卷 / 02期

关键词：

ALPHA-KETOGLUTARATE DEHYDROGENASE; HYSTERETIC PROPERTY; ALPHA-KETOGLUTARATE ANALOG; SH-GROUP; REGULATORY SITE;

D O I：

10.1016/0014-5793(91)80104-B

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The burst of product accumulation during the KGD reaction was investigated. It has been shown not to be the obligatory feature of catalysis, but appears when increasing the enzyme saturation by KG. Structural analogues of KG and the SH-group modification suppress the initial burst without preventing catalysis. The results obtained are in favour of the existence of the regulatory site for binding KG and its structural analogues essential for hysteretic properties of KGD.

引用

页码：147 / 150

页数：4

共 9 条

[1]

BEREZIN IV, 1976, PRACTICAL COURSE CHE

[2]

BUNIK V I, 1987, Biokhimiya, V52, P1235

[3]

BUNIK V I, 1985, Biokhimiya, V50, P1668

[4] CHANGE IN ALPHA-KETOGLUTARATE DEHYDROGENASE COOPERATIVE PROPERTIES DUE TO DIHYDROLIPOATE AND NADH [J].

BUNIK, VI ;

BUNEEVA, OA ;

GOMAZKOVA, VS .

FEBS LETTERS, 1990, 269 (01) :252-254

[5]

BUNIK VI, 1990, IN PRESS BIOCH INT

[6]

FRIEDEN C, 1970, J BIOL CHEM, V245, P5788

[7]

GOMAZKOVA V S, 1987, Biokhimiya, V52, P1144

[8]

RAY WJ, 1961, J BIOL CHEM, V236, P1973

[9]

SCRUTTON MC, 1965, J BIOL CHEM, V240, P3714

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