Cell-based assays and instrumentation for screening ion-channel targets

被引：123

作者：

Gonzalez, JE ^{[1
]}

Oades, K ^{[1
]}

Leychkis, Y ^{[1
]}

Harootunian, A ^{[1
]}

Negulescu, PA ^{[1
]}

机构：

[1] Aurora Biosci Corp, San Diego, CA 92121 USA

来源：

DRUG DISCOVERY TODAY | 1999年 / 4卷 / 09期

关键词：

D O I：

10.1016/S1359-6446(99)01383-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

ion channels are an important class of drug targets. They comprise the molecular basis for essential physiological functions including fluid secretion, electrolyte balance, bioenergetics and membrane excitabilily. High-throughput screening for ion-channel function requires sensitive, simple assays and instrumentation that will report ion channel activity in living cells. This article will review relevant assay technologies for ion channels and describe voltage-sensitive probes and instruments based on fluorescence resonance energy transfer (FRET) that enable ion-channel drug discovery.

引用

收藏

页码：431 / 439

页数：9

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