ANALYSIS OF UV-INDUCED DNA PHOTOPRODUCTS BY P-32 POSTLABELING

被引：36

作者：

BYKOV, VJ

KUMAR, R

FORSTI, A

HEMMINKI, K

机构：

[1] Center for Nutrition and Toxicology, Karolinska Institute, Novum

来源：

CARCINOGENESIS | 1995年 / 16卷 / 01期

关键词：

D O I：

10.1093/carcin/16.1.113

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

UV-induced cyclobutane dimers and 6-4 photoproducts, containing an;nmodified nucleotide at the 5'-position were released from DNA by means of digestion with DNase I, snake venom phosphodiesterase and prostatic acid phosphatase. The enzymes were deactivated by proteinase K followed by ethanol precipitation. The products were phosphorylated by polynucleotide kinase and [gamma-P-32]ATP. The TLC system used for the analysis enables separation of the different photoproducts and detection at a fmol level. T4 endonuclease treatment was applied to confirm the positions of cyclobutane dimers.

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页码：113 / 118

页数：6

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