DETECTION OF GLYCOPROTEINS IN BORRELIA-BURGDORFERI

被引：27

作者：

SAMBRI, V

STEFANELLI, C

CEVENINI, R

机构：

[1] UNIV BOLOGNA, S ORSOLA HOSP, INST MICROBIOL, I-40138 BOLOGNA, ITALY

[2] UNIV BOLOGNA, DEPT BIOCHEM, I-40138 BOLOGNA, ITALY

来源：

ARCHIVES OF MICROBIOLOGY | 1992年 / 157卷 / 03期

关键词：

BORRELIA-BURGDORFERI; GLYCOPROTEINS; DIGOXIGENIN LABELING; MONOCLONAL ANTIBODIES; N-GLYCOSIDASE-F; GEL FILTRATION HPLC;

D O I：

10.1007/BF00245150

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The presence of carbohydrates on proteins of Borrelia burgdorferi, the causative agent of Lyme disease, was investigated by using a digoxigenin labeling method together with Schiff staining and N-glycosidase F assay. The two major outer surface exposed proteins of 31 kDa and 34 kDa showed to be glycosylated and gel filtration high pressure liquid chromatography (HPLC) of proteins of B. burgdorferi metabolically labeled with C-14-N-acetylglucosamine revealed the incorporation of the carbohydrate into the glycosyl residue of these proteins.

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页码：205 / 208

页数：4

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