BREAKTHROUGH CURVE OF LYSINE ON A COLUMN OF A STRONG CATION-EXCHANGE RESIN OF THE AMMONIUM FORM

By using a "finite-segment" model for an ion-exchange column, breakthrough curves of ternary cation components - lysine, ammonium, and hydrogen - on a column of a cation-exchange resin of the ammonium form are calculated as a function of limited parameters such as the selectivity coefficients and the dissociation constant of the amino acid. Experimental breakthrough curves on a fixed column packed with Diaion SK-IB (nominal DVB 8%) are simulated and analyzed by the calculated results. Within a limited flow rate of the liquid phase, the model is valid for experimental results, including dependencies on the pH and the ratio of lysine to ammonium, when the conditions of electroneutrality are obeyed.