ASSOCIATION OF ICP0 BUT NOT ICP27 WITH PURIFIED VIRIONS OF HERPES-SIMPLEX VIRUS TYPE-1

被引：76

作者：

FENG, Y ^{[1
]}

COURTNEY, RJ ^{[1
]}

机构：

[1] LOUISIANA STATE UNIV,MED CTR,DEPT BIOCHEM & MOLEC BIOL,SHREVEPORT,LA 71130

来源：

JOURNAL OF VIROLOGY | 1992年 / 66卷 / 05期

关键词：

D O I：

10.1128/JVI.66.5.2709-2716.1992

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent studies have shown that ICP4, one of the major immediate-early proteins of herpes simplex virus type 1 is present within the tegument region of the virion (F. Yao and R.J. Courtney, J. Virol. 63:3338-3344, 1989). With monoclonal antibodies to two additional immediate-early proteins, ICP0 and ICP27, and Western blot (immunoblot) analysis, ICP0, but not ICP27, was also found to be associated with purified virus particles. In an effort to localize the ICP0 within the virion, purified virions were treated with trypsin in the presence and absence of detergent. The data suggest that ICP0 is located within the tegument region of the virion and is not localized in the envelope or within the nucleocapsid. The number of molecules of ICP0 per virion was estimated to be approximately 150.

引用

页码：2709 / 2716

页数：8

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