A C-13 NMR-STUDY OF THE APPLICATION OF [U-C-13]SUCCINATE FOR METABOLIC INVESTIGATIONS IN RABBIT RENAL PROXIMAL CONVOLUTED TUBULAR CELLS

被引：9

作者：

JANS, AWH ^{[1
]}

WILLEM, R ^{[1
]}

机构：

[1] VRIJE UNIV BRUSSELS,AOSC TW,B-1050 BRUSSELS,BELGIUM

来源：

MAGNETIC RESONANCE IN MEDICINE | 1990年 / 14卷 / 01期

关键词：

D O I：

10.1002/mrm.1910140114

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

The fate of the 13C label arising from [ U‐13C ] succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U‐13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of [U‐13C] succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of α‐ketoglutarate and oxaloacetate, respectively. © 1990 Academic Press, Inc. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

引用

页码：148 / 153

页数：6