A C-13 NMR-STUDY OF THE APPLICATION OF [U-C-13]SUCCINATE FOR METABOLIC INVESTIGATIONS IN RABBIT RENAL PROXIMAL CONVOLUTED TUBULAR CELLS

被引：9

作者：

JANS, AWH ^{[1
]}

WILLEM, R ^{[1
]}

机构：

[1] VRIJE UNIV BRUSSELS,AOSC TW,B-1050 BRUSSELS,BELGIUM

来源：

MAGNETIC RESONANCE IN MEDICINE | 1990年 / 14卷 / 01期

关键词：

D O I：

10.1002/mrm.1910140114

中图分类号：

R8 [特种医学]; R445 [影像诊断学];

学科分类号：

1002 ; 100207 ; 1009 ;

摘要：

The fate of the 13C label arising from [ U‐13C ] succinate in rabbit renal proximal convoluted tubular (PCT) cells, incubated with [U‐13C]succinate, was investigated by 13C NMR spectroscopy of the perchloric acid extracts of the cell pellets. The 13C label of [U‐13C] succinate was predominantly metabolized into glutamate, glutamine, and into glucose, lactate, and alanine, which is the consequence of a carbon efflux of the label from the Krebs cycle at the stage of α‐ketoglutarate and oxaloacetate, respectively. © 1990 Academic Press, Inc. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company

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页码：148 / 153

页数：6

相关论文

共 21 条

[1] IMPROVED RENAL CORTICAL TUBULE SUSPENSION - SPECTROPHOTOMETRIC STUDY OF O2 DELIVERY [J].

BALABAN, RS ;

SOLTOFF, SP ;

STOREY, JM ;

MANDEL, LJ .

AMERICAN JOURNAL OF PHYSIOLOGY, 1980, 238 (01) :F50-F59

[2] NATURAL-ABUNDANCE C-13 NMR OF BRAIN [J].

BARANY, M ;

ARUS, C ;

CHANG, YC .

MAGNETIC RESONANCE IN MEDICINE, 1985, 2 (03) :289-295

[3] NATURAL ABUNDANCE C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF LIVER AND ADIPOSE-TISSUE OF THE LIVING RAT [J].

CANIONI, P ;

ALGER, JR ;

SHULMAN, RG .

BIOCHEMISTRY, 1983, 22 (21) :4974-4980

[4] C-13 NMR-STUDIES OF GLUCONEOGENESIS IN RAT-LIVER CELLS - UTILIZATION OF LABELED GLYCEROL BY CELLS FROM EUTHYROID AND HYPER-THYROID RATS [J].

COHEN, SM ;

OGAWA, S ;

SHULMAN, RG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (04) :1603-1607

[5] C-13 NMR-STUDY OF EFFECTS OF FASTING AND DIABETES ON THE METABOLISM OF PYRUVATE IN THE TRICARBOXYLIC-ACID CYCLE AND OF THE UTILIZATION OF PYRUVATE AND ETHANOL IN LIPOGENESIS IN PERFUSED-RAT-LIVER [J].

COHEN, SM .

BIOCHEMISTRY, 1987, 26 (02) :581-589

[6]

COHEN SM, 1981, J BIOL CHEM, V256, P3428

[7] EFFECTS OF INSULIN ON PERFUSED LIVER FROM STREPTOZOTOCIN-DIABETIC AND UNTREATED RATS - C-13 NMR ASSAY OF PYRUVATE-KINASE FLUX [J].

COHEN, SM .

BIOCHEMISTRY, 1987, 26 (02) :573-580

[8] C-13 NMR-STUDIES OF INVIVO KINETIC RATES OF METABOLIC PROCESSES [J].

DENHOLLANDER, JA ;

SHULMAN, RG .

TETRAHEDRON, 1983, 39 (21) :3529-3538

[9]

DENHOLLANDER JA, 1981, P NATL ACAD SCI-BIOL, V78, P2693

[10] P-31 AND C-13 NUCLEAR MAGNETIC-RESONANCE STUDIES OF ANAEROBIC GLUCOSE-METABOLISM AND LACTATE TRANSPORT IN STAPHYLOCOCCUS-AUREUS CELLS [J].

EZRA, FS ;

LUCAS, DS ;

MUSTACICH, RV ;

RUSSELL, AF .

BIOCHEMISTRY, 1983, 22 (16) :3841-3849