RAPID, SINGLE-STEP DIFFERENTIATION OF EQUID HERPESVIRUS-1 AND HERPESVIRUS-4 FROM CLINICAL MATERIAL USING THE POLYMERASE CHAIN-REACTION AND VIRUS-SPECIFIC PRIMERS

被引：62

作者：

LAWRENCE, GL ^{[1
]}

GILKERSON, J ^{[1
]}

LOVE, DN ^{[1
]}

SABINE, M ^{[1
]}

WHALLEY, JM ^{[1
]}

机构：

[1] UNIV SYDNEY, DEPT VET PATHOL & BACTERIOL, SYDNEY, NSW 2006, AUSTRALIA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1994年 / 47卷 / 1-2期

基金：

澳大利亚研究理事会;

关键词：

EQUINE HERPESVIRUS; POLYMERASE CHAIN REACTION; DIAGNOSIS;

D O I：

10.1016/0166-0934(94)90066-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sets of primers were designed which enabled specific amplification of homologous regions of the glycoprotein C and gene 76 genetic loci of equine herpesviruses 1 and 4 (EHV-1 and EHV-4). The resultant virus-specific polymerase chain reaction (PCR) products arising from each loci could be discriminated easily on the basis of size on an agarose gel, allowing rapid differentiation of the two equine herpesviruses. Specificity of the amplifications were confirmed by Southern hybridization and restriction endonuclease digestion. The PCR test was applied to nasal swab samples from weanling foals and to archival aborted fetal tissue samples and the results compared to those obtained by virus Isolation. A strong correlation was found between this PCR assay and virus isolation methods of EHV-1 and EHV-4 detection and discrimination.

引用

页码：59 / 72

页数：14

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