Fluorescence Recovery after Photo-bleaching Apparatus Using Second Harmonic of 1120 nm Semiconductor Laser for Illumination

被引：0

作者：

Zhang, Z. L. ^{[1
]}

Hara, T. ^{[2
]}

Oguri, H. ^{[2
]}

Mo, Y. J. ^{[3
,4
]}

Aoyama, M. ^{[4
]}

Yoshida, H. ^{[4
]}

Kim, Y. H. ^{[1
]}

Rahman, Md. M. ^{[1
]}

Tero, R. ^{[4
]}

Urisu, T. ^{[1
,4
]}

机构：

[1] Grad Univ Adv Studies, Okazaki, Aichi 4448585, Japan

[2] Sumitomo Osaka Cemento Co Ltd, New Technol Res Labs, Funabashi, Chiba 2748601, Japan

[3] Henan Univ, Dept Phys, Kaifeng 475001, Peoples R China

[4] Inst Mol Sci, Okazaki, Aichi 4448585, Japan

来源：

E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY | 2005年 / 3卷

关键词：

Semiconductor laser; Second harmonic generation; Fluorescence recovery after photo-bleaching; FRAP; Lipid; Fluorescence microscope;

D O I：

10.1380/ejssnt.2005.254

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A compact fluorescence recovery after photo-bleaching (FRAP) apparatus was constructed using a semiconductor laser as an illumination light source. The second harmonic, 560 nm, of the 1120 nm semiconductor laser is guided to the light source point of the fluorescence microscope by an optical fiber. Since the illumination light wavelength 560 nm almost fits to the absorption maximum of the fluorescence dye molecule rhodamine B sulfonyl, the photo-bleaching was induced efficiently with minimum heating of the microscope lens system. The time dependence of the fluorescence recovery was directly measured by the photo-multiplier attached and the diffusion coefficient of the lipid molecules was calculated.

引用

页码：254 / 257

页数：4

共 9 条

[1] Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope [J].