ASSOCIATION OF MORPHOLOGICAL AND RFLP MARKERS IN RICE (ORYZA-SATIVA L)

被引:31
作者
YU, ZH
MCCOUCH, SR
KINOSHITA, T
SATO, S
TANKSLEY, SD
机构
[1] CORNELL UNIV,DEPT PLANT BREEDING & BIOMETRY,ITHACA,NY 14853
[2] HOKKAIDO UNIV,FAC AGR,SAPPORO,HOKKAIDO 060,JAPAN
[3] UNIV RYUKYUS,FAC AGR,OKINAWA 90301,JAPAN
关键词
LINKAGE MAPPING; MORPHOLOGICAL MARKER; RICE; ORYZA SATIVA L; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;
D O I
10.1139/g95-073
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Seventeen morphological marker genes were associated with restriction fragment length polymorphism markers in rice by using four F-2 populations, each segregating for a few observable traits, and 14 near isogenic lines (NILs), each containing one morphological mutant gene. The location of five genes was confirmed on the basis of F-2 analysis: Purple bull (Pr) (16.8 +/- 7.9 cM away from RG163 on chromosome 4); Phenol staining (Ph) (20.8 +/- 8.4 cM away from RG163 on chromosome 4); glabrous leaf and hull (gl-l) (14.3 +/- 7.4 cM away from RG182, and 20.9 +/- 8.3 cM from RG403 on chromosome 5); Brown pericarp (Re) (12.5 +/- 7.2 cM away from RG30 on chromosome 7); and lazy growth habit (la) (28.8 +/- 9.4 ci?A away from RG118 on chromosome 11). In addition, 12 other morphological markers, including the agronomically important genes semidwarf (sd-l) and Pollen restoring factor (Rf-l) were tentatively associated with mapped DNA clones based on screening pairs of NILs.
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页码:566 / 574
页数:9
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