ANTI-SYNTAXIN ANTIBODIES INHIBIT CALCIUM-DEPENDENT CATECHOLAMINE SECRETION FROM PERMEABILIZED CHROMAFFIN CELLS

被引：30
作者：
GUTIERREZ, LM
QUINTANAR, JL
VINIEGRA, S
SALINAS, E
MOYA, F
REIG, JA
机构：
[1] UNIV ALICANTE,FAC MED,DEPT NEUROQUIM,E-03080 ALACANT,SPAIN
[2] UNIV ALICANTE,FAC MED,DEPT GENET & MICROBIOL,E-03080 ALACANT,SPAIN
[3] UNIV ALICANTE,INST NEUROCIENCIAS,E-03080 ALACANT,SPAIN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 206卷 / 01期
关键词：
D O I：
10.1006/bbrc.1995.1001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adrenomedullary chromaffin cells release catecholamines in response to the intracellular calcium rise upon stimulation by different secretagogues. The presence of syntaxin 1, a protein presumably involved in docking of synaptic vesicles to presynaptic membranes, has been investigated in chromaffin cells. The study using two different monoclonal antibodies shows that syntaxin 1 is present in the chromaffin cell membrane fraction. Functional experiments demonstrate that anti-syntaxin antibodies inhibit calcium-dependent secretion in permeabilized cells. These results suggest that syntaxin 1 is an important component of the secretory machinery in chromaffin cells. (C) 1995 Academic Press, Inc.
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页码：1 / 7
页数：7
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