ION FLUXES ASSOCIATED WITH EXCITATORY AMINO-ACID-TRANSPORT

被引：474

作者：

WADICHE, JI ^{[1
]}

AMARA, SG ^{[1
]}

KAVANAUGH, MP ^{[1
]}

机构：

[1] OREGON HLTH SCI UNIV, HOWARD HUGHES MED INST, PORTLAND, OR 97201 USA

来源：

NEURON | 1995年 / 15卷 / 03期

关键词：

D O I：

10.1016/0896-6273(95)90159-0

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Flux of substrate and charge mediated by three cloned excitatory amino acid transporters widely expressed in human brain were studied in voltage-clamped Xenopus oocytes. Superfusion of L-glutamate or D-aspartate resulted in currents due in part to electrogenic Na+ cotransport, which contributed 1 net positive charge per transport cycle. A significant additional component of the currents was due to activation of a reversible anion flux that was not thermodynamically coupled to amino acid transport. The selectivity sequence of this ligand-activated conductance was NO3- > l(-) > Br- > Cl- > F-. The results suggest that these proteins mediate both transporter- and channel-like modes of permeation, providing a potential mechanism for dampening cell excitability, in addition to removal of transmitter.

引用

页码：721 / 728

页数：8

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