REFINED CONDITIONS FOR SELECTIVE MODIFICATIONS OF RABBIT SKELETAL MYOSIN LIGHT-CHAINS

被引：10

作者：

BURGAT, JM ^{[1
]}

ROULET, A ^{[1
]}

CARDINAUD, R ^{[1
]}

机构：

[1] UNIV PARIS 11,BIOL PHYS CHIM LAB,BAT 430,F-91405 ORSAY,FRANCE

来源：

BIOCHIMIE | 1992年 / 74卷 / 12期

关键词：

MYOSIN; ARTIFICIAL ISOFORMS; PROTEOLYSIS KINETICS; ATPASE STABILITY; CARDIOMYOPATHY;

D O I：

10.1016/0300-9084(92)90006-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We selectively modified the LC1 and LC2 N-terminus as an approach to understand the function of skeletal myosin light chains and their possible implication in some diseases. Three new myosin isoforms were thus created, namely: myosin-[(P)LC1'], myosin-[(T)LC2'] and myosin-[(CT)LC2''] in which the N-terminus was selectively cleaved at Lys7 in (P)LC1', Arg8 in (T)LC2' and Phe 19 in (CT)LC2''. In order to obtain species with a minimum amount of secondary cleavages, eight to 12 different conditions were screened for each species and the two most efficient conditions were tested at the preparative scale.

引用

页码：1083 / 1090

页数：8

共 33 条

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