CHARACTERIZATION OF INFECTIOUS TYPE-D RETROVIRUS FROM BABOONS

被引：25

作者：

GRANT, RF

WINDSOR, SK

MALINAK, CJ

BARTZ, CR

SABO, A

BENVENISTE, RE

TSAI, CC

机构：

[1] UNIV WASHINGTON,REG PRIMATE RES CTR,SEATTLE,WA 98195

[2] NCI,VIRAL CARCINOGENESIS LAB,FREDERICK,MD 21702

来源：

VIROLOGY | 1995年 / 207卷 / 01期

关键词：

D O I：

10.1006/viro.1995.1080

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Infectious virus resembling type D simian retrovirus (SRV) was isolated from Ethiopian baboons (Papio cynocephalus) (SRV-Pc) housed at the University of Washington Regional Primate Research Center. When baboon peripheral blood mononuclear cells (PBMC) or tissues were cocultured with the H-9 human T-cell line or the Raji human B-cell line, large multinucleated syncytia positive for sRV-2 antigens were observed microscopically. Immunoblot analysis of purified SRV-Pc from cell culture supernatants demonstrated that the viral core and envelope proteins reacted with rabbit anti-SRV-2 serum. Fresh PBMC and cocultured cells were positive by polymerase chain reaction using two different sets of SRV-2 primers. Preliminary sequence analysis of two separate isolates from portions of the SRV-Pc p27 and gp20 regions revealed homology with SRV-1, SRV-2, and Mason-Pfizer monkey virus. The homologies in the p27 segment were 91-94% and the homologies in the gp20 segment were 72-75%. (C) 1995 Academic Press, Inc.

引用

页码：292 / 296

页数：5

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