STABLE REGENERATION IN ASPARAGUS-COOPERI BAKER AS CONTROLLED BY DIFFERENT FACTORS

被引：8

作者：

GHOSH, B

SEN, S

机构：

[1] Centre of Advanced Study, Department of Botany, University of Calcutta, Calcutta, 700019, 35, Ballygunge Circular Road

来源：

PLANT SCIENCE | 1992年 / 82卷 / 01期

关键词：

ASPARAGUS-COOPERI; TISSUE CULTURE; EXPLANT TYPE; CALLUS; ORGANOGENESIS; PLANT REGENERATION; PHOTOPERIOD; CYTOLOGY;

D O I：

10.1016/0168-9452(92)90014-D

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

For in vitro regeneration spear, shoot tip, internode, node and root of mature plants of Asparagus cooperi were used as explants. Induction of callus from different explants depended on the photoperiod in addition to a specific combination of alpha-napthalene acetic acid (NAA) and kinetin (Kn) in the basal medium. The development of shoots from callus required 6-benzylaminopurine (BA), L-arginine, adenine and a low level of NAA. The individual shoots produced roots in the presence of indole-3-butyric acid (IBA) or indole-3-butyric acid containing potassium salt (KIBA). Regenerated plants were cytologically and phenotypically stable.

引用

页码：119 / 124

页数：6

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