AMINO-ACID SEQUENCE OF PORCINE INTESTINAL CALCIUM-BINDING PROTEIN

被引：97

作者：

HOFMANN, T ^{[1
]}

KAWAKAMI, M ^{[1
]}

HITCHMAN, AJW ^{[1
]}

HARRISON, JE ^{[1
]}

DORRINGTON, KJ ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT MED,TORONTO M5S 1A8,ONTARIO,CANADA

来源：

CANADIAN JOURNAL OF BIOCHEMISTRY | 1979年 / 57卷 / 06期

关键词：

D O I：

10.1139/o79-092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The complete amino acid sequence of the calcium-binding protein (CaBP) from pig intestinal mucosa has been determined: Ac-Ser-Ala-Gln-Lys-Ser-Pro-Ala-Glu-Leu-Lys-Ser-Ile-Phe-Glu-Lys-Tyr-Ala-Ala-Lys-Glu-Gly-Asp-Pro-Asn-Gln-Leu-Ser-Lys-Glu-Glu-Leu-Lys-Gln-Leu-Ile-Gln-Ala-Glu-Phe-Pro-Ser-Leu-Leu-Lys-Gly-Pro-Arg-Thr-Leu-Asp-Asp-Leu-Phe-Gln-Glu-Leu-Asp-Lys-Asn-Gly-Asn-Gly-Glu-Val-Ser-Phe-Glu-Glu-Phe-Gln-Val-Leu-Val-Lys-Lys-Ile-Ser-Gln-OH. The N-terminal octapeptide sequence was determined by mass spectrometric analysis by Morris and Dell. The first 45 residues of bovine CaBP differ only in six positions from the corresponding sequence of the porcine protein, except that the sequence starts in position two of the porcine sequence. The mammalian intestinal CaBP's belong to the troponin-C superfamily on the basis of an analysis by Barker and Dayhoff.

引用

页码：737 / 748

页数：12

共 41 条

[1]

BARKER WC, COMP BIOCH PHYSL

[2]

BARKER WC, 1977, CALCIUM BINDING PROT, P73

[3]

BARKER WC, 1979, ATLAS PROTEIN SEQ S3, V5, P273

[4] PRIMARY STRUCTURES OF N-TERMINAL EXTRA PEPTIDE SEGMENTS LINKED TO VARIABLE AND CONSTANT REGIONS OF IMMUNOGLOBULIN LIGHT CHAIN PRECURSORS - IMPLICATIONS ON ORGANIZATION AND CONTROLLED EXPRESSION OF IMMUNOGLOBULIN GENES [J].