CORN METABOLITES AFFECT GROWTH AND VIRULENCE OF AGROBACTERIUM-TUMEFACIENS

被引：91
作者：
SAHI, SV
CHILTON, MD
CHILTON, WS
机构：
[1] N CAROLINA STATE UNIV,DEPT BOT,RALEIGH,NC 27695
[2] CIBA GEIGY AGR BIOTECHNOL RES UNIT,RES TRIANGLE PK,NC 27709
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 10期
关键词：
2.4-dihydroxy-7-methoxy-2H-1,4-bezoxazin-3(4H)-one; Acetosyringone; corn transformation; growth inhibition; virulence gene induction;
D O I：
10.1073/pnas.87.10.3879
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Homogenates of corn seedlings inhibit both growth of Agrobacterium tumefaciens nd induction of its Ti plasmid virulence (vir) genes by acetosyringone (AS). The heat-liable inhibitor has been identified as 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA), present in 2-week-old seedlings (B73) at a concentration of 1.5 mM or greater. A concentration of 0.3 mM DIMBOA is sufficient to block growth of A. tumefaciens completely for 220 hr. DIMBOA at 0.1 mM concentration completely inhibited vir gene induction by 100 μM AS and reduced growth rate by 50%. Thus, DIMBOA can be expected to have a significant effect on attempts to transform corn by using A. tumefaciens as a vector.
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页码：3879 / 3883
页数：5
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