Water-fat magnetic resonance imaging quantifies relative proportions of brown and white adipose tissues: ex-vivo experiments

被引:4
作者
Stojanovska, Jadranka [1 ]
Lumeng, Carey N. [2 ]
Griffin, Cameron [3 ]
Hernando, Diego [4 ]
Hoffmann, Udo [5 ]
Haft, Jonathan W. [6 ]
Kim, Karen M. [6 ]
Burant, Charles F. [7 ]
Singer, Kanakadurga [8 ]
Tsodikov, Alex [9 ]
Long, Benjamin D. [10 ]
Romano, Matthew A. [11 ]
Tang, Paul C. [11 ]
Yang, Bo [11 ]
Chenevert, Thomas L. [12 ]
机构
[1] Michigan Med, Div Cardiothorac Radiol, Dept Radiol, Ann Arbor, MI 48109 USA
[2] Michigan Med, Dept Pediat & Mol Physiol, Ann Arbor, MI USA
[3] Michigan Med, Div Pediat Endocrinol, Ann Arbor, MI USA
[4] Univ Wisconsin, Dept Med Phys, Wisconsin Inst Med Res, 1530 Med Sci Ctr, Madison, WI 53706 USA
[5] Massachusetts Gen Hosp, Dept Radiol, Boston, MA USA
[6] Michigan Med, Dept Cardiac Surg, Frankel Cardiovasc Ctr, Ann Arbor, MI USA
[7] Univ Michigan, Ann Arbor, MI 48109 USA
[8] Michigan Med, Div Pediat Endocrinol, Dept Pediat & Communicable Dis, Ann Arbor, MI USA
[9] Sch Publ Hlth, Ann Arbor, MI USA
[10] Univ Michigan, Sch Med, Ctr Cardiovasc, Ann Arbor, MI USA
[11] Michigan Med, Cardiovasc Ctr, Ann Arbor, MI USA
[12] Michigan Med, Dept Radiol MRI, Ann Arbor, MI USA
关键词
multiecho Dixon; brown adipose tissue; white adipose tissue; ex-vivo study; T2*;
D O I
10.1117/1.JMI.5.2.024007
中图分类号
R8 [特种医学]; R445 [影像诊断学];
学科分类号
1002 ; 100207 ; 1009 ;
摘要
Quantifying the amount of brown adipose tissue (BAT) within white adipose tissue (WAT) in human depots may serve as a target to combat obesity. We aimed to quantify proton density fat fraction (PDFF) of BAT and WAT in relatively pure and in mixed preparation using water-fat imaging. Three ex-vivo experiments were performed at 3 T using excised interscapular BAT and inguinal/subcutaneous WAT from mice. The first two experiments consisted of BAT and WAT in separate tubes, and the third used mixed preparation with graded quantities of BAT and WAT. To investigate the influence of partial volume on PDFF metrics, low (2.66 mm(3)) and high spatial resolution (0.55 mm(3) acquired voxels) in two orthogonal three-dimensional sections were compared. The low-resolution acquisitions are corrected for T2* and multipeak lipid spectrum, thus considered "quantitative," whereas the high-resolution acquisitions are not corrected but were performed to better spatially segment BAT from WAT zones. As potential BAT metrics, we quantified the average PDFF and the volume of tissue having PDFF <= 50% (VOLPDFF <= 50%) based on the PDFF histogram. In the first experiment, the average PDFF of BAT was 23 +/- 6% and 21 +/- 7.6% and the average PDFF of WAT was 76 +/- 7% and 87 +/- 7% using high-and low-resolution techniques, respectively. A similar trend with excellent reproducibility in average PDFF of BAT and WAT was observed in the second experiment. In the third experiment over the four acquisitions, the BAT-dominant tube demonstrated lower PDFF (mean +/- SD) of 55 +/- 2% than WAT-dominant (69 +/- 4%) and WAT-only tubes (88 +/- 4%). Estimating VOLPDFF <= 50%, the BAT-dominant tube demonstrated higher volume of 0.26 cm(3) than WAT-dominant (0.16 cm(3)) and WAT-only tubes (0.01 cm(3)). The presence of BAT exhibits a lower PDFF relative to WAT, thus allowing segmentation of low PDFF tissue for quantification of volume representative of BAT. Future studies will determine the clinical relevance of BAT volume within human depots. (c) The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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