PURIFICATION OF CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II FROM RAT STOMACH AND ITS LOCALIZATION IN THE MUCOSA

被引：1
作者：
WANG, WJ
FUKUNAGA, K
GOTO, S
MIYAMOTO, E
机构：
[1] KUMAMOTO UNIV, SCH MED, DEPT PHARMACOL, KUMAMOTO 860, JAPAN
[2] KUMAMOTO UNIV, SCH MED, DEPT NEUROSURG, KUMAMOTO 860, JAPAN
来源：
BIOMEDICAL RESEARCH-TOKYO | 1994年 / 15卷 / 04期
关键词：
D O I：
10.2220/biomedres.15.231
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) with a M(r) of 530,000 was purified from rat stomach. The enzyme showed a major protein band with 52 kDa and minor components with 50 and 54 kD a when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The physicochemical and kinetic properties of the enzyme were similar to those of brain CaM kinase II. The enzyme showed a broad substrate specificity, and was capable of phosphorylating several endogenous proteins in the cytosol and membrane fractions. The polyclonal antibody against brain CaM kinase II cross-reacted with the enzyme. Strong immunoreactivity was observed in the parietal cells of stomach by the immunohistochemical investigation. These results suggest that CaM kinase II regulates the Ca2+ dependent cellular functions of the parietal cells.
引用
收藏
页码：231 / 240
页数：10
相关论文
共 50 条
[41]   Oligomerization of Ca2+/calmodulin-dependent protein kinase kinase [J].
Fukumoto, Yusei ;
Harada, Yuhei ;
Ohtsuka, Satomi ;
Kanayama, Naoki ;
Magari, Masaki ;
Hatano, Naoya ;
Sakagami, Hiroyuki ;
Tokumitsu, Hiroshi .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022, 587 :160-165
[42]   INCREASED PHOSPHORYLATION OF CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II AND ITS ENDOGENOUS SUBSTRATES IN THE INDUCTION OF LONG-TERM POTENTIATION [J].
FUKUNAGA, K ;
MULLER, D ;
MIYAMOTO, E .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (11) :6119-6124
[43]   OCCURRENCE AND ACTIVATION OF CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II AND ITS ENDOGENOUS SUBSTRATES IN BOVINE ADRENAL-MEDULLARY CELLS [J].
YANAGIHARA, N ;
TOYOHIRA, Y ;
YAMAMOTO, H ;
OHTA, Y ;
TSUTSUI, M ;
MIYAMOTO, E ;
IZUMI, F .
MOLECULAR PHARMACOLOGY, 1994, 46 (03) :423-430
[44]   PURIFICATION AND CHARACTERIZATION OF A MULTIFUNCTIONAL CALMODULIN-DEPENDENT PROTEIN-KINASE FROM RAT STOMACH [J].
WANG, WJ ;
FUKUNAGA, K ;
MIYAMOTO, E .
JAPANESE JOURNAL OF PHARMACOLOGY, 1987, 43 :P203-P203
[45]   Involvement of Ca2+/calmodulin-dependent protein kinase II in the spontaneous activation in rat oocytes [J].
不详 .
REPRODUCTION FERTILITY AND DEVELOPMENT, 2005, 17 (1-2) :279-280
[46]   CHARACTERIZATION OF A MONOMERIC KINASE-ACTIVITY FROM VASCULAR SMOOTH-MUSCLE - RELATIONSHIP TO CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II [J].
SCHWORER, CM ;
SWEELEY, CS ;
SINGER, HA .
FASEB JOURNAL, 1994, 8 (04) :A393-A393
[47]   Purification and characterization of a Ca2+/calmodulin-dependent protein kinase II from hog gastric mucosa using a protein-protein affinity chromatographic technique [J].
Fährmann, M ;
Möhlig, M ;
Schatz, H ;
Pfeiffer, A .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 255 (02) :516-525
[48]   REGULATION OF CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE-II BY INTERSUBUNIT-CATALYZED AND INTRASUBUNIT-CATALYZED AUTOPHOSPHORYLATIONS [J].
MUKHERJI, S ;
SODERLING, TR .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1994, 269 (19) :13744-13747
[49]   Phosphorylation at the nuclear localization signal of Ca2+/calmodulin-dependent protein kinase II blocks its nuclear targeting [J].
Heist, EK ;
Srinivasan, M ;
Schulman, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (31) :19763-19771
[50]   Immunohistochemical localization of Ca2+/calmodulin-dependent kinase in tobacco [J].
Yang, WN ;
Liang, SP ;
Lu, YT .
CHINESE SCIENCE BULLETIN, 2001, 46 (03) :230-233
12345