TOPOGRAPHY OF THE ESCHERICHIA-COLI RIBOSOMAL 30S SUBUNIT-INITIATION FACTOR-II COMPLEX

被引：10
作者：
WAKAO, H
ROMBY, P
EBEL, JP
GRUNBERGMANAGO, M
EHRESMANN, C
EHRESMANN, B
机构：
[1] CNRS,INST BIOL MOLEC & CELLULAIRE,15 RUE R DESCARTES,F-67084 STRASBOURG,FRANCE
[2] INST BIOL PHYS CHIM,F-75005 PARIS,FRANCE
来源：
BIOCHIMIE | 1991年 / 73卷 / 7-8期
关键词：
ESCHERICHIA-COLI; INITIATION FACTOR-IF2; 16S RIBOSOMAL-RNA; STRUCTURE PROBING; CROSS-LINKING;
D O I：
10.1016/0300-9084(91)90140-V
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The specific effect of the binding of initiation factor IF2 on E coli 16S rRNA within the [IF2/30S/GTP] complex has been probed by crosslinking experiment with trans-diamminedichloro platinum (II) and by phosphate alkylation with ethylnitrosourea. Several 16S rRNA fragments crosslinked to IF2 have been identified and are mostly located in the head and the lateral protrusion of the 30S subunit. The study of the effect of IF2 binding to the 30S subunit reveals that the factor does not tightly bind to the 16S rRNA and induces both isolated reductions and enhancements of phosphate reactivity in the 16S rRNA. Several of them are located near the binding site of IF2 and weak effects are observed in distant parts of the subunit. These results are discussed in the light of current knowledge of the topographical localization of IF2 with the 30S subunit and of its relation with function.
引用
收藏
页码：991 / 1000
页数：10
相关论文
共 46 条
[1]   CROSSLINKING OF TRANSCRIPTION FACTOR TFIIA TO RIBOSOMAL 5S RNA FROM X-LAEVIS BY TRANS-DIAMMINEDICHLOROPLATINUM(II) [J].
BAUDIN, F ;
ROMBY, P ;
ROMANIUK, PJ ;
EHRESMANN, B ;
EHRESMANN, C .
NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :10035-10046
[2]   HIGHER-ORDER STRUCTURE OF DOMAIN-III IN ESCHERICHIA-COLI 16S RIBOSOMAL-RNA, 30S SUBUNIT AND 70S RIBOSOME [J].
BAUDIN, F ;
EHRESMANN, C ;
ROMBY, P ;
MOUGEL, M ;
COLIN, J ;
LEMPEREUR, L ;
BACHELLERIE, JP ;
EBEL, JP ;
EHRESMANN, B .
BIOCHIMIE, 1987, 69 (10) :1081-1096
[3]   PROBING THE PHOSPHATES OF THE ESCHERICHIA-COLI RIBOSOMAL 16S RNA IN ITS NAKED FORM, IN THE 30S SUBUNIT, AND IN THE 70S RIBOSOME [J].
BAUDIN, F ;
MOUGEL, M ;
ROMBY, P ;
EYERMANN, F ;
EBEL, JP ;
EHRESMANN, B ;
EHRESMANN, C .
BIOCHEMISTRY, 1989, 28 (14) :5847-5855
[4]   DIRECT CROSS-LINKS BETWEEN INITIATION FACTOR-I, FACTOR-II, AND FACTOR-III AND RIBOSOMAL-PROTEINS PROMOTED BY 2-IMINOTHIOLANE [J].
BOILEAU, G ;
BUTLER, P ;
HERSHEY, JWB ;
TRAUT, RR .
BIOCHEMISTRY, 1983, 22 (13) :3162-3170
[5]  
BOLLEN A, 1975, J BIOL CHEM, V250, P4310
[6]   A DETAILED MODEL OF THE 3-DIMENSIONAL STRUCTURE OF ESCHERICHIA-COLI 16-S RIBOSOMAL-RNA INSITU IN THE 30-S SUBUNIT [J].
BRIMACOMBE, R ;
ATMADJA, J ;
STIEGE, W ;
SCHULER, D .
JOURNAL OF MOLECULAR BIOLOGY, 1988, 199 (01) :115-136
[7]   MECHANISM OF TRANSLATIONAL INITIATION IN PROKARYOTES - EVIDENCE FOR A DIRECT EFFECT OF IF2 ON THE ACTIVITY OF THE 30-S RIBOSOMAL-SUBUNIT [J].
CANONACO, MA ;
CALOGERO, RA ;
GUALERZI, CO .
FEBS LETTERS, 1986, 207 (02) :198-204
[8]   A COMPLETE MAPPING OF THE PROTEINS IN THE SMALL RIBOSOMAL-SUBUNIT OF ESCHERICHIA-COLI [J].
CAPEL, MS ;
ENGELMAN, DM ;
FREEBORN, BR ;
KJELDGAARD, M ;
LANGER, JA ;
RAMAKRISHNAN, V ;
SCHINDLER, DG ;
SCHNEIDER, DK ;
SCHOENBORN, BP ;
SILLERS, IY ;
YABUKI, S ;
MOORE, PB .
SCIENCE, 1987, 238 (4832) :1403-1406
[9]   PROTEIN-RNA CROSSLINKING IN ESCHERICHIA-COLI 30S RIBOSOMAL-SUBUNITS - IDENTIFICATION OF A 16S RIBOSOMAL-RNA FRAGMENT CROSSLINKED TO PROTEIN-S12 BY THE USE OF THE CHEMICAL CROSSLINKING REAGENT 1-ETHYL-3-DIMETHYL-AMINOPROPYLCARBODIIMIDE [J].
CHIARUTTINI, C ;
EXPERTBEZANCON, A ;
HAYES, D ;
EHRESMANN, B .
NUCLEIC ACIDS RESEARCH, 1982, 10 (23) :7657-7676
[10]   IF-3 CROSSLINKING TO ESCHERICHIA-COLI RIBOSOMAL 30-S SUBUNITS BY 3 DIFFERENT LIGHT-DEPENDENT PROCEDURES - IDENTIFICATION OF 30-S-PROTEINS CROSSLINKED TO IF-3 - UTILIZATION OF A NEW 2-STAGE CROSSLINKING REAGENT, PARA-NITROBENZYLMALEIMIDE [J].
COOPERMAN, BS ;
EXPERTBEZANCON, A ;
KAHAN, L ;
DONDON, J ;
GRUNBERGMANAGO, M .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1981, 208 (02) :554-562
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