ALTERED GROWTH OF HUMAN COLON CANCER CELL-LINES DISRUPTED AT ACTIVATED KI-RAS

被引：593

作者：

SHIRASAWA, S ^{[1
]}

FURUSE, M ^{[1
]}

YOKOYAMA, N ^{[1
]}

SASAZUKI, T ^{[1
]}

机构：

[1] KYUSHU UNIV,MED INST BIOREGULAT,DEPT GENET,3-1-1 MAIDASHI,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

SCIENCE | 1993年 / 260卷 / 5104期

关键词：

D O I：

10.1126/science.8465203

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Point mutations that activate the Ki-ras proto-oncogene are present in about 50 percent of human colorectal tumors. To study the functional significance of these mutations, the activated Ki-ras genes in two human colon carcinoma cell lines, DLD-1 and HCT 116, were disrupted by homologous recombination. Compared with parental cells, cells disrupted at the activated Ki-ras gene were morphologically altered, lost the capacity for anchorage-independent growth, grew more slowly both in vitro and in nude mice, and showed reduced expression of c-myc. Thus, the activated Ki-ras gene plays a key role in colorectal tumorigenesis through altered cell differentiation and cell growth.

引用

页码：85 / 88

页数：4

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