SYNTHETIC PEPTIDES TO IDENTIFY ANTIGENIC DETERMINANTS ON EPSTEIN-BARR-VIRUS GP350/220

被引：7
作者：
BERTONI, G
KOSTYAL, DA
REISERT, PS
HUMPHREYS, RE
SAIRENJI, T
机构：
[1] UNIV MASSACHUSETTS,SCH MED,DEPT PHARMACOL,55 LAKE AVE N,WORCESTER,MA 01655
[2] UNIV MASSACHUSETTS,SCH MED,DEPT MED,WORCESTER,MA 01605
来源：
INTERVIROLOGY | 1990年 / 31卷 / 05期
关键词：
Antipeptide antibodies; Epstein-Barr virus; Gp350/220 synthetic peptides;
D O I：
10.1159/000150164
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We synthesized three peptides, MAI - Thr19-Val28(+ Tyr) -, MA2 - Ser807-Ala816-, and MA3 - Ser718-Glu729(+Tyr) from the sequence of Epstein-Barr virus gp350/220 and immunized rabbits with these peptides. Rabbit antisera to the peptides had antipeptide radioimmunoassay titers of 1:400 foranti-MA1, 1:200 foranti-MA2, and 1:1600 foranti-MA3. The anti-MAI serum recognized gp350/220 in Western blotting to SDS-electrophoresed proteins from l2-0-tetradecanoylphorbol-l3-acetate- and n-butyrate-treated B95-8 cells, but anti-MA2 and MA3 sera did not. None of the sera reacted with gp350/220 by membrane or cytoplasmic immunonuorescence or by immunoprecipitation of Triton X-100 solubilized proteins. © 1990 S. Karger AG, Basel.
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收藏
页码：290 / 294
页数：5
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