DNA FRAGMENTATION INDICATIVE OF APOPTOSIS FOLLOWING UNILATERAL CEREBRAL HYPOXIA-ISCHEMIA IN THE NEONATAL RAT

被引：146

作者：

HILL, IE ^{[1
]}

MACMANUS, JP ^{[1
]}

RASQUINHA, I ^{[1
]}

TUOR, UI ^{[1
]}

机构：

[1] NAT RES COUNCIL,INST BIODIAGNOST,WINNIPEG,MB R3B 1Y6,CANADA

来源：

BRAIN RESEARCH | 1995年 / 676卷 / 02期

关键词：

APOPTOSIS; HYPOXIA-ISCHEMIA; INTERNUCLEOSOMAL CLEAVAGE; NEONATAL; DNA DAMAGE; CELL DEATH;

D O I：

10.1016/0006-8993(95)00145-G

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

DNA extracted from regional brain samples of hypoxic/ischemic neonatal rats showed internucleosomal cleavage indicative of apoptosis. Cells containing cleaved DNA were identified by in situ labelling in the cortex, hippocampus, striatum and thalamus of the ipsilateral hemisphere. When the effects of increasing the length of the hypoxia were examined, increases were seen in the amount of internucleosomally cleaved DNA and in the number of labelled cells.

引用

页码：398 / 403

页数：6

共 31 条

[1]

Buja, Eigenbrodt, Eigenbrodt, Apoptosis and necrosis: basic types and mechanisms of cell death, Arch. Pathol. Lab. Med., 117, pp. 1208-1214, (1993)

[2]

Deckwerth, Johnson, Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor, J. Cell Biol., 123, pp. 1207-1222, (1993)

[3]

Deshpande, Bergstedt, Linden, Kalimo, Wieloch, Ultrastructural changes in the hippocampal CA1 region following transient cerebral ischemia evidence against programmed cell death, Exp. Br. Res., 88, pp. 91-105, (1992)

[4]

Diana, Setzu, Sirigu, Diaz, Nuclear patterns of apoptotic and developing neurons of superior cervical ganglion of newborn rat, Int. J. Dev. Neurosci., 11, pp. 773-780, (1993)

[5]

Edwards, Tolkovsky, Characterization of apoptosis in cultured rat sympathetic neurons after nerve growth factor withdrawal, J. Cell Biol., 124, pp. 537-546, (1994)

[6]

Ferrer, Tortosa, Macaya, Sierra, Moreno, Munell, Blanco, Squier, Evidence of nuclear DNA fragmentation following hypoxia-ischemia in the infant rat brain, and transient forebrain ischemia in the adult gerbil, Br. Pathol., 4, pp. 115-122, (1994)

[7]

Forloni, Angaretti, Chiesa, Monzani, Salmona, Bugiani, Tagliavini, Neurotoxicity of a prion protein fragment, Nature (London), 362, pp. 543-546, (1993)

[8]

Forloni, Chiesa, Smiroldo, Verga, Salmona, Tagliavini, Angeretti, Apoptosis mediated neurotoxicity induced by chronic application of β amyloid fragment 25–35, NeuroReport, 4, pp. 523-526, (1993)

[9]

Garcia, Lossinsky, Kauffman, Conger, Neuronal ischemic injury: light microscopy, ultrastructure and biochemistry, Acta Neuropathol. (Berlin), 43, pp. 85-95, (1978)

[10]

Gavrieli, Sherman, Ben-Sasson, Identification of programmed cell death in situ via specific labelling of nuclear DNA fragmentation, J. Cell Biol., 119, pp. 493-501, (1992)

← 1 2 3 4 →