TRANSFORMATION OF MYCOPLASMA-PNEUMONIAE WITH TN4001 BY ELECTROPORATION

被引：66

作者：

HEDREYDA, CT ^{[1
]}

LEE, KK ^{[1
]}

KRAUSE, DC ^{[1
]}

机构：

[1] UNIV GEORGIA,DEPT MICROBIOL,ATHENS,GA 30602

来源：

PLASMID | 1993年 / 30卷 / 02期

关键词：

D O I：

10.1006/plas.1993.1047

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Mycoplasma pneumoniae was transformed with the Staphylococcus aureus transposon Tn4001 by electroporation. A transformation frequency of 10-3 to 10-5/colony-forming unit was observed using 30.0 μg plasmid DNA and 107-108M. pneumoniae colony-forming units. DNA hybridization analyses using standard and pulsed field agarose gel electrophoresis confirmed chromosomal insertion of the transposon, apparently by a transpositional mechanism into random sites. These studies demonstrate the functionality of Tn4001 in M. pneumoniae and suggest its potential as a genetic tool in this mycoplasma. © 1993 Academic Press. All rights reserved.

引用

页码：170 / 175

页数：6

共 28 条

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