Dataset reporting detection of breast cancer-related HER2(I655V) polymorphism using allele-specific polymerase chain reaction

The dataset presented in this article is related to the research article entitled "Detection of HER2 Gene Polymorphism in Breast Cancer: PCR Optimization Study" (B.R. Budiarto, Desriani, 2016) [1] with some modification in primers used and in PCR optimization strategy to eliminate false-positive result that may occur in HERI655V polymorphism detection. Combining a new set of primers with PCR gradient, The allele-specific PCR well performs to detect all type of breast cancer-originated HER2(I655V) genotypes. The validation of this method was done using Sanger DNA sequencing, offering an alternative tool for HERI655V polymorphism detection in another type of cancer. (C) 2016 The Authors. Published by Elsevier Inc.