ALUMINUM IN TEA - SEC-ICP-MS SPECIATION STUDIES OF INFUSIONS AND SIMULATED GASTROINTESTINAL DIGESTS

被引:30
作者
OWEN, LMW [1 ]
CREWS, HM [1 ]
MASSEY, RC [1 ]
机构
[1] MAFF,FOOD SCI LAB,FOOD SAFETY DIRECTORATE,NORWICH NR4 7UQ,ENGLAND
来源
CHEMICAL SPECIATION AND BIOAVAILABILITY | 1992年 / 4卷 / 03期
基金
英国医学研究理事会;
关键词
SIZE EXCLUSION CHROMATOGRAPHY; INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY; ALUMINUM SPECIATION; TEA INFUSION; INVITRO GASTROINTESTINAL DIGESTION;
D O I
10.1080/09542299.1992.11083185
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The speciation of aluminium in tea infusions and in vitro gastrointestinal digests of tea infusions has been investigated using size exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS). At pH 2.5, following simulated gastric treatment, Al from tea eluted at a similar retention volume to that obtained for an aqueous Al standard. At pH 5.5, an aqueous Al standard was eluted from an SEC column in Tris buffer (Al recovery almost-equal-to 100%) only in the presence of a complexing agent (NaF), and at a retention volume corresponding to a molecular mass greater than that expected tor an ionic species. Aluminium associated with a tea infusion eluted in two tractions: a higher molecular weight fraction corresponding to Al strongly bound to ligands in the tea, and a lower molecular weight fraction probably comprised of labile Al eluting as Al-F complexes. Simulated gastrointestinal digestion produced three Al-bearing fractions, of which the two at higher molecular mass represented ligand-bound Al. The molarity of the Tris buffer strongly influenced the retention volume of the Al fractions, particularly for the ligand-bound Al. After simulated gastrointestinal digestion, the soluble labile fraction (15% of the Al from the tea infusion) was considered to be potentially available for absorption. The actual proportion of the fraction that might be absorbed would depend upon a number of physiological and nutritional factors.
引用
收藏
页码:89 / 96
页数:8
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