REGULATION OF THE ADHE GENE, WHICH ENCODES ETHANOL DEHYDROGENASE IN ESCHERICHIA-COLI

被引：28

作者：

CHEN, YM ^{[1
]}

LIN, ECC ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DEPT MICROBIOL & MOLEC GENET,200 LONGWOOD AVE,BOSTON,MA 02115

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 24期

关键词：

D O I：

10.1128/jb.173.24.8009-8013.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The respiratory control of adhE, which encodes ethanol (alcohol) dehydrogenase in Escherichia coli, was examined at the transcriptional level by using various PHI(adhE'-lacZ) adhE+ merodiploid strains. Expression of the adhE'-lacZ operon fusion was increased about eightfold by anaerobic growth. Under anaerobic growth conditions, provision of nitrate lowered the level of expression. Nitrate repression was more severe than aerobic repression. From analyses of various mutants with mutations related to nitrate reduction, nitrate repression appears to result from two effects. (i) When nitrate was present, NarL, the positive regulator of the nar operon, exerted a direct repression on adhE expression, which was demonstrable even aerobically. (ii) The chemical reduction of nitrate exerted an indirect effect by altering the cellular redox potential.

引用

页码：8009 / 8013

页数：5

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