STUDIES ON HUMAN AND BOVINE PLATELET MEMBRANE-GLYCOPROTEINS

1. 1. We have re-evaluated the number of platelet membrane glycoproteins with use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and two highly sensitive detection techniques. 2. 2. The first involves incorporation of the fluorophore dansyi hydrazine into the carbohydrate moiety after post-electrophoresis periodate oxidation. 3. 3. The second involves tritium labelling of galactosyl residues in the intact platelet, electrophoresis of membrane proteins, and autoradiography. 4. 4. In each case more glycoproteins were detected with these procedures than with periodate-Schiffs base stain. 5. 5. The human platelet membrane contains about 16 resolvable glycoprotein components in the 35,000-300,000 molecular weight range and the bovine platelet membrane contains about 17. © 1979.