CELL-CYCLE AND CELL-SIZE DEPENDENCE OF SUSCEPTIBILITY TO HYDRODYNAMIC-FORCES

被引:36
作者
ALRUBEAI, M
SINGH, RP
EMERY, AN
ZHANG, Z
机构
关键词
CELL CYCLE; HYDRODYNAMIC FORCES; APOPTOSIS; CELL CULTURE;
D O I
10.1002/bit.260460112
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Exposure of animal cells to intense hydrodynamic forces exerted in turbulent capillary flow, and by controlled agitation and aeration, resulted in preferential destruction of S and G(2) cells and the extent of destruction of these cells was dependent upon the intensity of the action. The loss of these cells was possibly due to their larger size. However, the appearance of large numbers of membrane-bound vesicular structures similar to apoptotic bodies as well as cells with low DNA stainability (in a sub-G(1) peak) suggested that the action of adverse hydrodynamic forces on these large cells may at least in part be to induce an apoptotic response. (C) 1995 John Wiley and Sons, Inc.
引用
收藏
页码:88 / 92
页数:5
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