ENDOCYTOSIS AND INTRACELLULAR DEGRADATION OF HETEROLOGOUS PROTEIN BY EOSINOPHILIC GRANULOCYTES ISOLATED FROM RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) POSTERIOR INTESTINE

被引：15

作者：

DORIN, D ^{[1
]}

SIRE, MF ^{[1
]}

VERNIER, JM ^{[1
]}

机构：

[1] UNIV PARIS 11, PHYSIOL CELLULAIRE & METAB POISSONS LAB, F-91405 ORSAY, FRANCE

来源：

BIOLOGY OF THE CELL | 1993年 / 79卷 / 03期

关键词：

TROUT; EGC; PROTEIN ENDOCYTOSIS; IN VITRO STUDY;

D O I：

10.1016/0248-4900(93)90140-A

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Adherence capacity to tissue substrate, endocytosis capacity for heterologous and homologous proteins, and proteolytic activity were determined in intestinal granulocytes (EGCs) isolated from healthy adult rainbow trout. The percentage of cells that could adhere to a smooth plastic surface increased with increasing incubation time. Endocytosis was effective for heterologous (human immunoglobulin G, IgGh; ovine somatotropin, oST) but not homologous proteins (recombinant trout somatotropin, rtST). The activity of cathepsin D increased significantly after the endocytosis of a heterologous protein. Finally, the analysis of immunoblots of homogenates of granulocytes incubated in the presence of the two different proteins was used to show the endocytosis and degradation of heterologous proteins. These results show that isolated EGCs can endocytose and degrade heterologous proteins.

引用

页码：219 / 224

页数：6

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