STRUCTURE OF A NOVEL INSP3 RECEPTOR

被引:366
|
作者
SUDHOF, TC [1 ]
NEWTON, CL [1 ]
ARCHER, BT [1 ]
USHKARYOV, YA [1 ]
MIGNERY, GA [1 ]
机构
[1] UNIV TEXAS,SW MED CTR,HOWARD HUGHES MED INST,DALLAS,TX 75235
来源
EMBO JOURNAL | 1991年 / 10卷 / 11期
关键词
CA2+ CHANNEL; ENDOPLASMIC RETICULUM; INTRACELLULAR CA2+; RYANODINE RECEPTOR; SIGNAL TRANSDUCTION;
D O I
10.1002/j.1460-2075.1991.tb04882.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inositol 1,4,5-trisphosphate (InsP3) constitutes a major intracellular second messenger that transduces many growth factor and neurotransmitter signals. InsP3 causes the release of Ca2+ from intracellular stores by binding to specific receptors that are coupled to Ca2+ channels. One such receptor from cerebellum has previously been extensively characterized. We have now determined the full structure of a second, novel InsP3 receptor which we refer to as type 2 InsP3 receptor as opposed to the cerebellar type 1 InsP3 receptor. The type 2 InsP3 receptor has the same general structural design as the cerebellar type 1 InsP3 receptor with which it shares 69% sequence identity. Expression of the amino-terminal 1078 amino acids of the type 2 receptor demonstrates high affinity binding of InsP3 to the type 2 receptor with a similar specificity but higher affinity than observed for the type 1 receptor. These results demonstrate the presence of several types of InsP3 receptor in brain and raise the possibility that intracellular Ca2+ signaling may involve multiple pathways with different regulatory properties dependent on different InsP3 receptors.
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页码:3199 / 3206
页数:8
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