ANALYSIS OF THE SITE OF ACTION OF THE AMDR PRODUCT FOR REGULATION OF THE AMDS GENE OF ASPERGILLUS-NIDULANS

被引：44

作者：

LITTLEJOHN, TG ^{[1
]}

HYNES, MJ ^{[1
]}

机构：

[1] UNIV MELBOURNE,DEPT GENET,PARKVILLE,VIC 3052,AUSTRALIA

来源：

MOLECULAR & GENERAL GENETICS | 1992年 / 235卷 / 01期

关键词：

ASPERGILLUS-NIDULANS; GENE REGULATION; CCAAT BOX; AMDR REGULATORY GENE; PROMOTER;

D O I：

10.1007/BF00286184

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The amdR gene of Aspergillus nidulans regulates a number of structural genes in response to omega amino acid inducers. The site of action of the amdR product on expression of the amdS gene was investigated by studying the effects of changes in the 5' region of amdS, generated in vitro, on the induction, and on responses of an amdS-lacZ fusion gene to an amdR(c) allele. A sequence was identified that is sufficient for amdR regulation and that shows identity with sequences involved in amdR regulation of the gatA and lam genes. This sequence includes a CCAAT sequence and it was shown that this sequence is an important element in setting the basal level of amdS expression.

引用

页码：81 / 88

页数：8

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