CHROMATOGRAPHIC-SEPARATION OF THE SOLUBLE-PROTEINS OF HENS EGG-YOLK - ANALYTICAL AND PREPARATIVE STUDY

被引：28
|
作者：
BURLEY, RW
VADEHRA, DV
机构：
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 94卷 / 01期
关键词：
D O I：
10.1016/0003-2697(79)90789-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gel filtration chromatography on columns of Ultragel in 1.0 M NaCl was used to fractionate the soluble proteins of the yolk of Australorp hen''s eggs. Five fractions were isolated quantitatively: 3 major fractions each about a third of the total soluble protein by weight and each consisting largely of 1 of the 3 known proteins, .alpha.-, .beta.- and .gamma.-livetin, and 2 minor fractions neither more than 3% of the total. One minor fraction contained a single protein of low molecular weight, apovitellenin II, previously isolated from the yolk low-density lipoprotein. The other, referred to as .delta.-livetin, is of very high molecular weight. Recoveries of the soluble proteins were affected by factors such as concentrated salt, organic solvents, or heating.
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页码：53 / 59
页数：7
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