CHROMATOGRAPHY OF P-32-LABELLED OLIGONUCLEOTIDES ON THIN LAYERS OF DEAE-CELLULOSE

被引：487
作者：
BROWNLEE, GG
SANGER, F
机构：
[1] M.R.C. Laboratory of Molecular Biology, Cambridge, CB2 2QH, Hills Road
来源：
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 11卷 / 02期
关键词：
D O I：
10.1111/j.1432-1033.1969.tb00786.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A two‐dimensional fractionation procedure has been developed for separating radioactively‐labelled oligonucleotides of up to 50 residues long, using uniformly 32P‐labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate at pH 3.5 in the first dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE‐cellulose and cellulose in the second dimension. Copyright © 1969, Wiley Blackwell. All rights reserved
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页码：395 / &
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