EFFECT OF DIETHYLETHER UPON FUNCTION OF VESICLES OF SARCOPLASMIC RETICULUM

被引：74

作者：

FIEHN, W

HASSELBA.W

机构：

[1] Max-Planck-Institut Für Medizinische Forschung, Heidelberg, BRD-69

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1969年 / 9卷 / 04期

关键词：

D O I：

10.1111/j.1432-1033.1969.tb00647.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The calcium permeability of the sarcoplasmic vesicles is greatly increased when vesicular suspensions are extracted with ether. A 7–8% v/v aqueous ether solution abolishes the calcium concentrating ability of the vesicles completely. The calcium remaining in the solution permanently activates the calcium magnesium dependent ATPase. In parallel to the change of the permeability of the vesicular membranes membranal lipids are removed by the ether treatment. Concentrations of ether which produce a maximal permeability increase only extract cholesterol esters. Higher concentrations of ether which inhibit the transport ATPase remove neutral lipids and phospholipids. The basic ATPase is not affected. Copyright © 1969, Wiley Blackwell. All rights reserved

引用

页码：574 / &

共 15 条

[1]

BAIRD CD, 1960, BIOCHEM J, V77, P262

[2] BINDING OF CALCIUM TRANSPORT INHIBITORS RESERPINE CHLORPROMAZINE AND PRENYLAMINE TO LIPIDS OF MEMBRANES OF SARCOPLASMIC RETICULUM [J].

BALZER, H ;

MAKINOSE, M ;

FIEHN, W ;

HASSELBACH, W .

NAUNYN-SCHMIEDEBERGS ARCHIV FUR PHARMAKOLOGIE, 1968, 260 (05) :456-+

[3] QUANTITATIVE THIN LAYER CHROMATOGRAPHY OF LIPIDS [J].

CUZNER, ML ;

DAVISON, AN .

JOURNAL OF CHROMATOGRAPHY, 1967, 27 (02) :388-+

[4]

EBERHAGEN DIETRICH, 1963, Z PHYSIOL CHEM [HOPPE SEYLER S], V333, P179

[5]

FIEHN W, UNPUBLISHED OBSERVAT

[6] ATP AND ACTIVE TRANSPORT [J].

HASSELBACH, W ;

MAKINOSE, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1962, 7 (02) :132-+

[7]

HASSELBACH W, 1963, BIOCHEM Z, V339, P94

[8]

HASSELBACH W, 1966, BIOCHEM Z, V345, P159

[9]

HOLMAN RT, 1966, PROGRESS CHEMISTRY F, V9, P1

[10]

INESI G, 1967, J BIOL CHEM, V242, P4637

← 1 2 →