DETERMINATION OF OPTICAL PURITY BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY UPON NONCHIRAL STATIONARY PHASES WITH DUAL CIRCULAR DICHROISM/ABSORPTION DETECTION

被引：31

作者：

BERTUCCI, C ^{[1
]}

SALVADORI, P ^{[1
]}

GUIMARAES, LFL ^{[1
]}

机构：

[1] CNR,CTR STUDIO MACROMOLEC STEREORDINATE OTTICAMENTE A,DIPARTIMENTO CHIM & CHIM IND,I-56126 PISA,ITALY

来源：

JOURNAL OF CHROMATOGRAPHY A | 1994年 / 666卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(94)80415-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of a circular dichroism (CD)-based detection system in HPLC using a non-chiral stationary phase is presented. The simultaneous measurement of CD, absorbance and anisotropy factor allows the determination of optical purity and the assignment of the absolute configuration to the prevailing enantiomer. This represents a further application of CD to analyse an optically active compound. The reliability of the method and the detection limit depend on the value of the anisotropy factor and on the Cd intensity, respectively.

引用

页码：535 / 539

页数：5

共 15 条

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