FLUORESCENCE INSITU HYBRIDIZATION FOR DIRECT VISUALIZATION OF GRAM-NEGATIVE ANAEROBES IN SUBGINGIVAL PLAQUE SAMPLES

被引:0
作者
GERSDORF, H [1 ]
PELZ, K [1 ]
GOBEL, UB [1 ]
机构
[1] ALBERT LUDWIGS UNIV KLINIKUM,INST MED MIKROBIOL & HYG,FREIBURG,GERMANY
来源
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1993年 / 6卷 / 2-3期
关键词
PERIODONTITIS; PORPHYROMONAS-GINGIVALIS; BACTEROIDES-FORSYTHUS; INSITU HYBRIDIZATION; 16S RIBOSOMAL-RNA; MICROBIAL ECOLOGY;
D O I
暂无
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
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页码:109 / 114
页数:6
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