RECOGNITION OF UUN CODONS BY 2 LEUCINE TRANSFER-RNA SPECIES FROM ESCHERICHIA-COLI

被引：11
作者：
TAKAI, K
HORIE, N
YAMAIZUMI, Z
NISHIMURA, S
MIYAZAWA, T
YOKOYAMA, S
机构：
[1] UNIV TOKYO,GRAD SCH SCI,DEPT BIOPHYS & BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN
[2] NATL CANC CTR,RES INST,DIV BIOL,CHUO KU,TOKYO 104,JAPAN
来源：
FEBS LETTERS | 1994年 / 344卷 / 01期
关键词：
CODON RECOGNITION; MODIFIED NUCLEOSIDE; PROTEIN SYNTHESIS (IN VITRO); AMINOACYL-TRANSFER-RNA COMPETITION; CONFORMATIONAL; RIGIDITY; RESTRICTED WOBBLING;
D O I：
10.1016/0014-5793(94)00354-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Codon recognition by Escherichia coli tRNA(4)(Leu) and tRNA(5)(Leu) was investigated by analysis of the competition between two aminoacyl-tRNA species in an in vitro protein synthesis. Both tRNA species strictly obey the wobble rule when they are in competition with other tRNA species. This is probably due to the post-transcriptional modifications at the first position of the anticodon of these tRNA(Leu) species, supporting the proposal that the conformational rigidity of post-transcriptionally modified pyrimidine nucleotides guarantees the correct codon recognition.
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页码：31 / 34
页数：4
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