Data on partial polyhydroxyalkanoate synthase genes (phaC) mined from Aaptos aaptos marine sponge-associated bacteria metagenome

被引:2
|
作者
Amelia, Tan Suet May [1 ]
Amirul, Al Ashraf Abdullah [2 ,3 ,4 ]
Bhubalan, Kesaven [1 ,3 ,5 ]
机构
[1] Univ Malaysia Terengganu, Sch Marine & Environm Sci, Kuala Nerus 21030, Terengganu, Malaysia
[2] Univ Sains Malaysia, Sch Biol Sci, George Town 11800, Malaysia
[3] NIBM, Malaysian Inst Pharmaceut & Nutraceut, George Town 11700, Malaysia
[4] Sains USM, Ctr Chem Biol, First Floor,Block B,10,Persiaran Bukit Jambul, Bayan Lepas 11900, Penang, Malaysia
[5] Univ Malaysia Terengganu, Inst Marine Bioltechnol, Kuala Nerus 21030, Terengganu, Malaysia
来源
DATA IN BRIEF | 2018年 / 16卷
关键词
Marine sponge; Metagenome; Polyhydroxyalkanoate synthase gene; Phylogenetic analysis;
D O I
10.1016/j.dib.2017.11.011
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report data associated with the identification of three polyhydroxyalkanoate synthase genes (phaC) isolated from the marine bacteria metagenome of Aaptos aaptos marine sponge in the waters of Bidong Island, Terengganu, Malaysia. Our data describe the extraction of bacterial metagenome from sponge tissue, measurement of purity and concentration of extracted metagenome, polymerase chain reaction (PCR)-mediated amplification using degenerate primers targeting Class I and II phaC genes, sequencing at First BASE Laboratories Sdn Bhd, and phylogenetic analysis of identified and known phaC genes. The partial nucleotide sequences were aligned, refined, compared with the Basic Local Alignment Search Tool (BLAST) databases, and released online in GenBank. The data include the identified partial putative phaC and their GenBank accession numbers, which are Rhodocista sp. phaC (MF457754), Pseudomonas sp. phaC (MF437016), and an uncultured bacterium AR5-9d_16 phaC (MF457753). (C) 2017 The Authors. Published by Elsevier Inc.
引用
收藏
页码:75 / 80
页数:6
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