EXPRESSION OF THE CHICKEN BETA-GLOBIN GENE-CLUSTER IN MICE - CORRECT DEVELOPMENTAL EXPRESSION AND DISTRIBUTED CONTROL

To investigate the regulation of gene clusters, we introduced the entire chicken beta-globin cluster into mice. This 35-kb region includes the four globin genes (rho-beta(H)-beta(A)-epsilon), the four upstream hypersensitive sites, and the intergenic beta(A)/epsilon enhancer. The chicken globins are not arranged in order of developmental expression, which is unlike the case for the human beta-globin cluster, in which gene order plays a role in the regulation of globin expression. Mice carrying the chicken cluster expressed the transgenes,vith the same developmental patterns as seen in the chicken. Therefore, stage-specific erythroid transcriptional milieus existed before the divergence of birds and mammals and have been conserved since then. Mice bearing the complete cluster except for a deletion removing the beta(A)/epsilon enhancer displayed markedly reduced expression of the beta(H), beta(A) and epsilon genes with efficient (but variable) rho expression. Mice carrying the four genes and beta(A)/epsilon enhancer but without the upstream hypersensitive sites showed reduced expression of rho, beta(H), and beta(A), with variable expression of epsilon. We conclude that (i) all of the genes (except possibly rho) are under the control of both the upstream hypersensitive sites and the enhancer, (ii) the influence of the control elements can extend beyond the nearest active gene, (iii) a single element (the enhancer) can influence more than one gene in a single developmental stage, (iv) the enhancer can work bidirectionally, and (v) neither the upstream sites (as a group) nor the enhancer showed developmental stage specificity. Thus, the regulation of this cluster is achieved by interaction of two distinct control regions with each of the globin genes.