CERVICAL-CARCINOMA - LOW-FREQUENCY OF ALLELE LOSS AT LOCI IMPLICATED IN OTHER COMMON MALIGNANCIES

Twenty cervical carcinomas were examined for loss of heterozygosity (LOH) using 22 RFLP markers, which mapped to regions of putative oncosuppressor gene loci, identified as candidates in other common solid tumours. Allele losses were identified in six of the eight chromosomal arms examined, but at a significantly lower frequency than that reported in other common solid tumours. No association was observed between allele losses at any chromosomal location and the presence or integration of 'high risk' types of HPV determined by a sensitive, specific PCR method. HPV 16, 18 or 33 were found in the majority (75%) of these tumours. We have looked at only a limited subset of chromosomal regions, but the results, so far, imply that carcinoma of the cervix may arise by different molecular events than other common solid tumours. and support the view that one of the distinctive events may be infection with HPV. Alternatively, similar molecular events may be occurring, but in regions of the genome not yet identified as targets in other solid tumours.