IDENTIFICATION AND CHARACTERIZATION OF THE LANTIBIOTIC NISIN-Z, A NATURAL NISIN VARIANT

被引：322

作者：

MULDERS, JWM ^{[1
]}

BOERRIGTER, IJ ^{[1
]}

ROLLEMA, HS ^{[1
]}

SIEZEN, RJ ^{[1
]}

DEVOS, WM ^{[1
]}

机构：

[1] NETHERLANDS INST DAIRY RES NIZO, DEPT BIOPHYS CHEM, POB 20, 6710 BA EDE, NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 201卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb16317.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lactococcus lactis strain NIZO 22186 produces an extracellular, lanthionine-containing 3.5-kDa polypeptide with antimicrobial activity. Its retention time on reversed-phase (RP) HPLC and its amino acid composition showed high similarities but no complete identity to nisin. The gene for this lantibiotic, designated nisZ, has been cloned and its nucleotide sequence was found to be identical to that of the precursor nisin gene apart from a single mutation resulting in the substitution His27Asn in the mature polypeptide. NMR studies of the natural nisin variant, which has been designated nisin Z, confirmed the His27Asn substitution and indicated that it has a similar structure to nisin.

引用

页码：581 / 584

页数：4

共 18 条

[1]

BANERJEE S, 1988, J BIOL CHEM, V263, P9508

[2]

BUCHMAN GW, 1988, J BIOL CHEM, V263, P16260

[3] CONFIRMATION OF THE STRUCTURE OF NISIN BY COMPLETE H-1-NMR RESONANCE ASSIGNMENT IN AQUEOUS AND DIMETHYL-SULFOXIDE SOLUTION [J].

CHAN, WC ;

LIAN, LY ;

BYCROFT, BW ;

ROBERTS, GCK .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1989, (12) :2359-2367

[4] ANALYSIS OF THE GENETIC DETERMINANT FOR PRODUCTION OF THE PEPTIDE ANTIBIOTIC NISIN [J].

DODD, HM ;

HORN, N ;

GASSON, MJ .

JOURNAL OF GENERAL MICROBIOLOGY, 1990, 136 :555-566

[5]

Gross E, 1977, Adv Exp Med Biol, V86B, P131

[6] STRUCTURE OF NISIN [J].

GROSS, E ;

MORELL, JL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1971, 93 (18) :4634-+

[7] PRESENCE OF DEHYDROALANINE IN ANTIBIOTIC NISIN AND ITS RELATIONSHIP TO ACTIVITY [J].

GROSS, E ;

MORELL, JL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1967, 89 (11) :2791-&

[8]

Hurst A, 1981, NISIN ADV APPL MICRO, V27, P85, DOI [10.1016/S0065-2164(08)70342-3, DOI 10.1016/S0065-2164(08)70342-3]

[9] NISIN, A PEPTIDE ANTIBIOTIC - CLONING AND SEQUENCING OF THE NISA GENE AND POSTTRANSLATIONAL PROCESSING OF ITS PEPTIDE PRODUCT [J].

KALETTA, C ;

ENTIAN, KD .

JOURNAL OF BACTERIOLOGY, 1989, 171 (03) :1597-1601

[10] GALLIDERMIN - A NEW LANTHIONINE-CONTAINING POLYPEPTIDE ANTIBIOTIC [J].

KELLNER, R ;

JUNG, G ;

HORNER, T ;

ZAHNER, H ;

SCHNELL, N ;

ENTIAN, KD ;

GOTZ, F .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 177 (01) :53-59

← 1 2 →