PRODUCTION OF INDOLE ALKALOIDS BY SURFACE IMMOBILIZED C-ROSEUS CELLS

被引：14

作者：

ARCHAMBAULT, J ^{[1
]}

VOLESKY, B ^{[1
]}

KURZ, WGW ^{[1
]}

机构：

[1] MCGILL UNIV,DEPT CHEM ENGN,BIOCHEM ENGN RES UNIT,MONTREAL H3A 2A7,QUEBEC,CANADA

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1990年 / 35卷 / 07期

关键词：

D O I：

10.1002/bit.260350703

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A technique was developed to surface immobilize plant cells and was scaled up in laboratory size bioreactors. This technique was shown not to hinder the biosynthetic potential of Catharanthus roseus immobilized cells and to induce a partial release (300 μg/L) of serpentine into the culture medium contrary to suspension cultured cells. The release pattern seemed to follow the biosynthesis trends of the product. This release mechanism could be stimulated by a factor of 10 within 2 h by increasing the pH of the culture from 5.0 to 5.5. Copyright © 1990 John Wiley & Sons, Inc.

引用

页码：660 / 667

页数：8

共 15 条

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