A RAPID HPLC METHOD TO SEPARATE THE TRIPLET PROTEINS OF NEUROFILAMENT

被引：35

作者：

KARLSSON, JE

ROSENGREN, LE

HAGLID, KG

机构：

[1] Institute of Neurobiology, University of Goteborg, Goteborg

来源：

JOURNAL OF NEUROCHEMISTRY | 1987年 / 49卷 / 05期

关键词：

HPLC; Intermediate filaments; Neurofilament; Purification; Separation;

D O I：

10.1111/j.1471-4159.1987.tb01002.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: In this article a fast HPLC technique to separate the individual neurofilament proteins is described. Highly pure fractions of the three neurofilament proteins can be obtained. As much as 50 mg of each neurofilament poly‐peptide can be separated from a crude neurofilament protein preparation in one step in less than 2 h. The short separation time is of importance in minimizing degradation, especially of the 150‐kilodalton neurofilament poly‐peptide. Copyright © 1987, Wiley Blackwell. All rights reserved

引用

页码：1375 / 1378

页数：4

共 16 条

[1]

DAHL D, 1977, J COMP NEUROL, V176, P645, DOI 10.1002/cne.901760412

[2] STUDY OF THE 10-NM-FILAMENT FRACTION ISOLATED DURING THE STANDARD MICROTUBULE PREPARATION [J].