Semi-nested polymerase chain reaction-based detection of Babesia spp. in small ruminants from Northwest of Iran

Aim: The present study aimed to detect Babesia ovis and Babesia motasi in the blood samples of sheep and goats from Northwest of Iran by the semi-nested polymerase chain reaction (PCR) teclmique. Materials and Methods: A total of 166 whole blood samples (including 123 sheep and 43 goats) were collected. In the first stage, the PCR was performed to amplify a piece of 18S rRNA gene of Babesia and Theileria genera. Then. semi-nested PCR was carried out on all PCR products to differentiate B. ovis and B. motasi. Results: The PCR indicated that totally, 119 (11.44%) out of 166 samples were positive for Babesia or Theileria spp. The semi-nested PCR showed that 38 samples (22.89%) were positive only for B. ovis. No significant association was found between the infection rate of B. ovis and age, gender and species of animals. Conclusion: In the present study, there was no evidence for B. motasi infection in small ruminants from Northwest of Iran. Therefore. B. ovis was the main causative agent of ovine Babesiosis in this region.